Quantitative X-ray microanalysis of hydrogen peroxide within plant cells.
Identifieur interne : 003509 ( Main/Exploration ); précédent : 003508; suivant : 003510Quantitative X-ray microanalysis of hydrogen peroxide within plant cells.
Auteurs : Shaoliang Chen [République populaire de Chine] ; Andrea Olbrich ; Rosemarie Langenfeld-Heyser ; Eberhard Fritz ; Andrea PolleSource :
- Microscopy research and technique [ 1097-0029 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Peroxyde d'hydrogène.
- composition chimique : Espace intracellulaire, Feuilles de plante, Populus.
- méthodes : Coloration et marquage.
- Cérium, Hydroxydes, Microanalyse par sonde électronique, Phosphates.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Hydrogen Peroxide.
- chemical : Cerium, Hydroxides, Phosphates.
- chemistry : Intracellular Space, Plant Leaves, Populus.
- methods : Staining and Labeling.
- Electron Probe Microanalysis.
Abstract
Using quantitative X-ray microanalysis in combination with CeCl3-based cytochemical staining of hydrogen peroxide (H2O2) we have developed a new solution for quantification of H2O2 at the subcellular level. Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv. showed that the obtained cerium precipitates by CeCl3 staining were the mixture of cerium perhydroxides and cerium phosphate, in which the fractions of CePO4 were: (1) 52-74% in cell walls of fresh leaf segments, and (2) 34-70% in the cytoplasm in 10 mM H2O2-treated leaf segments that were previously freeze-dried. Taking the concentration of cerium phosphate as staining background, we reached the cellular concentration of cerium perhydroxides and the corresponding concentration of H2O2. Results showed that H2O2 was present in the cytoplasm of rehydrated leaf segments (29-58 mM), but in fresh leaves, H2O2 was observed in the walls of all measured cell types (17-74 mM).
DOI: 10.1002/jemt.20639
PubMed: 18837436
Affiliations:
Links toward previous steps (curation, corpus...)
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantitative X-ray microanalysis of hydrogen peroxide within plant cells.</title><author><name sortKey="Chen, Shaoliang" sort="Chen, Shaoliang" uniqKey="Chen S" first="Shaoliang" last="Chen">Shaoliang Chen</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. lschen@bjfu.edu.cn</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Olbrich, Andrea" sort="Olbrich, Andrea" uniqKey="Olbrich A" first="Andrea" last="Olbrich">Andrea Olbrich</name></author><author><name sortKey="Langenfeld Heyser, Rosemarie" sort="Langenfeld Heyser, Rosemarie" uniqKey="Langenfeld Heyser R" first="Rosemarie" last="Langenfeld-Heyser">Rosemarie Langenfeld-Heyser</name></author><author><name sortKey="Fritz, Eberhard" sort="Fritz, Eberhard" uniqKey="Fritz E" first="Eberhard" last="Fritz">Eberhard Fritz</name></author><author><name sortKey="Polle, Andrea" sort="Polle, Andrea" uniqKey="Polle A" first="Andrea" last="Polle">Andrea Polle</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:18837436</idno><idno type="pmid">18837436</idno><idno type="doi">10.1002/jemt.20639</idno><idno type="wicri:Area/Main/Corpus">003782</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003782</idno><idno type="wicri:Area/Main/Curation">003782</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003782</idno><idno type="wicri:Area/Main/Exploration">003782</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Quantitative X-ray microanalysis of hydrogen peroxide within plant cells.</title><author><name sortKey="Chen, Shaoliang" sort="Chen, Shaoliang" uniqKey="Chen S" first="Shaoliang" last="Chen">Shaoliang Chen</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. lschen@bjfu.edu.cn</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Olbrich, Andrea" sort="Olbrich, Andrea" uniqKey="Olbrich A" first="Andrea" last="Olbrich">Andrea Olbrich</name></author><author><name sortKey="Langenfeld Heyser, Rosemarie" sort="Langenfeld Heyser, Rosemarie" uniqKey="Langenfeld Heyser R" first="Rosemarie" last="Langenfeld-Heyser">Rosemarie Langenfeld-Heyser</name></author><author><name sortKey="Fritz, Eberhard" sort="Fritz, Eberhard" uniqKey="Fritz E" first="Eberhard" last="Fritz">Eberhard Fritz</name></author><author><name sortKey="Polle, Andrea" sort="Polle, Andrea" uniqKey="Polle A" first="Andrea" last="Polle">Andrea Polle</name></author></analytic><series><title level="j">Microscopy research and technique</title><idno type="eISSN">1097-0029</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cerium (MeSH)</term><term>Electron Probe Microanalysis (MeSH)</term><term>Hydrogen Peroxide (analysis)</term><term>Hydroxides (MeSH)</term><term>Intracellular Space (chemistry)</term><term>Phosphates (MeSH)</term><term>Plant Leaves (chemistry)</term><term>Populus (chemistry)</term><term>Staining and Labeling (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Coloration et marquage (méthodes)</term><term>Cérium (MeSH)</term><term>Espace intracellulaire (composition chimique)</term><term>Feuilles de plante (composition chimique)</term><term>Hydroxydes (MeSH)</term><term>Microanalyse par sonde électronique (MeSH)</term><term>Peroxyde d'hydrogène (analyse)</term><term>Phosphates (MeSH)</term><term>Populus (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hydrogen Peroxide</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cerium</term><term>Hydroxides</term><term>Phosphates</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Peroxyde d'hydrogène</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Intracellular Space</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Espace intracellulaire</term><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Coloration et marquage</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electron Probe Microanalysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cérium</term><term>Hydroxydes</term><term>Microanalyse par sonde électronique</term><term>Phosphates</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Using quantitative X-ray microanalysis in combination with CeCl3-based cytochemical staining of hydrogen peroxide (H2O2) we have developed a new solution for quantification of H2O2 at the subcellular level. Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv. showed that the obtained cerium precipitates by CeCl3 staining were the mixture of cerium perhydroxides and cerium phosphate, in which the fractions of CePO4 were: (1) 52-74% in cell walls of fresh leaf segments, and (2) 34-70% in the cytoplasm in 10 mM H2O2-treated leaf segments that were previously freeze-dried. Taking the concentration of cerium phosphate as staining background, we reached the cellular concentration of cerium perhydroxides and the corresponding concentration of H2O2. Results showed that H2O2 was present in the cytoplasm of rehydrated leaf segments (29-58 mM), but in fresh leaves, H2O2 was observed in the walls of all measured cell types (17-74 mM).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18837436</PMID><DateCompleted><Year>2009</Year><Month>01</Month><Day>29</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1097-0029</ISSN><JournalIssue CitedMedium="Internet"><Volume>72</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Microscopy research and technique</Title><ISOAbbreviation>Microsc Res Tech</ISOAbbreviation></Journal><ArticleTitle>Quantitative X-ray microanalysis of hydrogen peroxide within plant cells.</ArticleTitle><Pagination><MedlinePgn>49-60</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/jemt.20639</ELocationID><Abstract><AbstractText>Using quantitative X-ray microanalysis in combination with CeCl3-based cytochemical staining of hydrogen peroxide (H2O2) we have developed a new solution for quantification of H2O2 at the subcellular level. Quantitative X-ray microanalysis of plastic-embedded leaves of Populus euphratica Oliv. showed that the obtained cerium precipitates by CeCl3 staining were the mixture of cerium perhydroxides and cerium phosphate, in which the fractions of CePO4 were: (1) 52-74% in cell walls of fresh leaf segments, and (2) 34-70% in the cytoplasm in 10 mM H2O2-treated leaf segments that were previously freeze-dried. Taking the concentration of cerium phosphate as staining background, we reached the cellular concentration of cerium perhydroxides and the corresponding concentration of H2O2. Results showed that H2O2 was present in the cytoplasm of rehydrated leaf segments (29-58 mM), but in fresh leaves, H2O2 was observed in the walls of all measured cell types (17-74 mM).</AbstractText><CopyrightInformation>Copyright 2008 Wiley-Liss, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shaoliang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China. lschen@bjfu.edu.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Olbrich</LastName><ForeName>Andrea</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Langenfeld-Heyser</LastName><ForeName>Rosemarie</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Fritz</LastName><ForeName>Eberhard</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Polle</LastName><ForeName>Andrea</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Microsc Res Tech</MedlineTA><NlmUniqueID>9203012</NlmUniqueID><ISSNLinking>1059-910X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006878">Hydroxides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010710">Phosphates</NameOfSubstance></Chemical><Chemical><RegistryNumber>30K4522N6T</RegistryNumber><NameOfSubstance UI="D002563">Cerium</NameOfSubstance></Chemical><Chemical><RegistryNumber>85885-77-4</RegistryNumber><NameOfSubstance UI="C048873">cerium hydroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>TH8E3IE00V</RegistryNumber><NameOfSubstance UI="C026690">cerous chloride</NameOfSubstance></Chemical><Chemical><RegistryNumber>XR1Z4BIW2J</RegistryNumber><NameOfSubstance UI="C048083">cerium phosphate</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002563" MajorTopicYN="N">Cerium</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004577" MajorTopicYN="Y">Electron Probe Microanalysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006878" MajorTopicYN="N">Hydroxides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042541" MajorTopicYN="N">Intracellular Space</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010710" MajorTopicYN="N">Phosphates</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18837436</ArticleId><ArticleId IdType="doi">10.1002/jemt.20639</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Fritz, Eberhard" sort="Fritz, Eberhard" uniqKey="Fritz E" first="Eberhard" last="Fritz">Eberhard Fritz</name><name sortKey="Langenfeld Heyser, Rosemarie" sort="Langenfeld Heyser, Rosemarie" uniqKey="Langenfeld Heyser R" first="Rosemarie" last="Langenfeld-Heyser">Rosemarie Langenfeld-Heyser</name><name sortKey="Olbrich, Andrea" sort="Olbrich, Andrea" uniqKey="Olbrich A" first="Andrea" last="Olbrich">Andrea Olbrich</name><name sortKey="Polle, Andrea" sort="Polle, Andrea" uniqKey="Polle A" first="Andrea" last="Polle">Andrea Polle</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Shaoliang" sort="Chen, Shaoliang" uniqKey="Chen S" first="Shaoliang" last="Chen">Shaoliang Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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